Author(s):
1. Marija Radojković, Univerzitet u Novom Sadu, Tehnološki fakultet, Novi Sad, Serbia
2. Simone Morais, Portugal
3. Cristina Soares, Portugal
4. Fátima Barroso, Portugal
5. Manuela Moreira, Portugal
6. Aleksandra Cvetanović, Serbia
7. Jaroslava Švarc-Gajić, Serbia
8. Milena Vujanović, Serbia
9. Cristina Delerue-Matos, Portugal

Abstract:
Teucrium chamaedrys L. is a perennial herbaceous plant very popular in folk medicine in Western Europe, Balkan and Middle East regions. Preparations and extracts have shown wide range of biological activity against various disease and conditions. In this study the microwave-assisted extraction (MAE) of phenolic compounds from T. chamaedrys L. and the phenolic composition and antioxidant activity of the obtained extracts were investigated. A 23 factorial design coupled with response surface methodology (RSM) was used. The optimized MAE conditions were ethanol: water (1:1; v/v), medium stirring speed, 120°C, 32 min, and 0.029 g sample/mL solvent. The statistical analysis gave that linear and quadratic terms as temperature, extraction time, sample-to-solvent ratio were the most significant parameters of the extraction process (p<0.01). Under the MAE optimized conditions, the maximum TPC was 81.3±6.9 mg GAE/g dry plant (DP), antioxidant activity was 111.2±5.3 mg AA/g DP (FRAP assay), 86.3±2.2 mg TX/g DP (DPPH assay) and 328.3±28.5 mg TE/g DP (ORAC assay). When compared to conventional extraction, MAE presented higher yields. Based on these results of investigation, it could be concluded that extracts of T. chamaedrys are a rich source of phenolic compounds as natural antioxidants of high value.

Key words:
Teucrium chamaedrys L., microwave-assisted extraction, response surface methodology, phenolic compounds

Thematic field:
Food Technology

Date of abstract submission:
16.07.2016.

Conference:
XI Savjetovanje hemičara, tehnologa i ekologa Republike Srpske

Abstract